Fig. 1

Overview of Oncogene Concatenated Enriched Amplicon Nanopore Sequencing (OCEANS) approach. (a) Short DNA amplicons from genes or loci of interest potentially bearing somatic mutations (red rectangle) have a lower throughput and low confidence somatic variant calls without using OCEANS. (b) We first use the blocker displacement amplification (BDA) technology to selectively amplify DNA sequence variants, so that somatic mutations with low sample VAF (≤5%) are represented in high VAF in the prepared DNA library. (c) Subsequently, we enzymatically concatenate the amplicons to increase the effective throughput of Nanopore Sequencing. OCEANS exhibits roughly 100-fold better mutation VAF limit of detection and roughly 10-fold higher throughput compared to the short amplicon SQK-LSK109 Nanopore dataset generated without using OCEANS