Fig. 2

(A) Upper part: Schematic representation of the types of splicing events analyzed in this study. Lower part: Schematic representation of short-read assignment to the reference transcriptome for skipping exon events. (B) Bar plot depicting the number of different splicing events that follow the expected pattern of expression of NMD targets. (C) Pie chart representing the percentage of novel (identified by long-read cDNA sequencing) and previously known exons (contained in the previous annotation) that are represented in the augmented annotation. (D) Pie chart showing the sets of exons found to respond to SMG6 or SMG7 KD. (E) Scatter plot depicting the log₂ fold change (log₂FC) of NMD-sensitive transcripts (y-axis) versus the log₂FC between dKD and CTR of their corresponding genes (x-axis) showing alternative exon events (Ν= 8'056) based on SMG6/SMG7 double KD, CTR KD and SMG6 Rescue conditions