Fig. 5

The deTOKI-predicted single-cell TAD-like domains characterize cell identity. The classification of single cells based on predicted TAD-like domain boundaries in Tan’s, Li’s, and Flyamer’s datasets are shown in a–c, respectively. Each dot represents a cell. The x- and y-axis represent the PC1 calculated by deTOKI and IS, respectively. The embedded plots show the AUC of the classification by each program. Cell #16 is colored in green in panel a, as it was in M/G1 phase. d The correlation of DNA methylation between bin pairs. The “inter-TAD-like domain” indicates that the two bins are separated by a predicted TAD-like domain boundary, while the “intra-TAD-like domain” indicates that the two bins belong to the same predicted TAD-like domain. Only bins fulfilling the criteria of (1) being members of pairs separated by 240 kb and (2) having reads containing a total of 2 or more CpGs, were included in this plot. *P < 0.05, **P < 0.001, NS: not significant, Fisher’s z-test [68]