Fig. 2

Splice junction discovery in human lung (HLCA) and mouse lemur lung (MLCA) cells. A SICILIAN filters out a higher proportion of unannotated junctions than annotated in all individuals from both human and mouse Lemur datasets [only junctions with at least two reads in the given dataset are plotted]. B Splice junctions identified by SICILIAN in gene GDI1 in human; annotated splicing was maintained and unannotated noisy junctions were all removed. C Better discrimination between annotated and unannotated junctions in the HLCA dataset achieved by the SICILIAN statistical criterion. D The number of junctions found in both human individuals that are called consistently by SICILIAN is larger than the number that are called differently in almost every case, regardless of the number of junctional reads. E The number of junctions called consistently between the two mouse lemur individuals is also larger than the number called inconsistently at almost every read depth after SICILIAN filtering. F The fraction of HLCA junctions that are found in CHESS and GTEx databases before and after applying SICILIAN to STAR raw calls. G The number of mouse lemur junctions orthologous junctions (found by LiftOver from Mmur3 to hg38) that have been also detected in the HLCA dataset. Junctions have been further classified based on their annotation status in the mouse lemur and human transcriptomes