Fig. 8
From: The dynamics of N6-methyladenine RNA modification in interactions between rice and plant viruses
Rice m6A methylation levels are positively associated with the expression of key genes involved in antiviral RNA silencing pathways and plant hormone signals. A Comparisons of m6A methylation levels of the respect mock-treated and RBSDV- and RSV-infected rice plants at 0, 1, 2, 4, 8, and 16 dpi by LC-MS/MS. Error bars indicate mean ± SD, with three biological replicates. B Dot-blot analysis of m6A levels in extracted total RNA from samples at 16 dpi using the specific anti-m6A antibodies. The left side of the membrane depicts the amount of loaded mRNA from mock-treated and RBSDV- and RSV-infected rice plants, respectively. C qRT-PCR analysis of the relative expression of OsAGO18 in mock-treated and RBSDV- and RSV-infected rice plants at 0, 1, 2, 4, 8, and 16 dpi. D Relative expression of the OsSLRL1 in the three treatments at 0, 1, 2, 4, 8, and 16 dpi. E Analysis of m6A methylation levels on different fragments of OsAGO18 by m6A-IP-qPCR. The upper panel indicates the gene structures of OsAGO18 labelled with fragments amplified in the m6A-IP-qPCR assay. The results of positions 1 and 12 were chosen for figure exhibition. F m6A-IP-qPCR assay of the m6A methylation levels of different fragments on OsSLRL1. Similarly, the upper panel represents the gene structures labelled with fragments amplified in the m6A-IP-qPCR analyses. Results of positions 2, 3, and 4 were selected for the display in the figure. Error bars denote mean ± SD, n = 3 biological replicates in all qRT-PCR assays