Fig. 2

Simulation study results. A Characteristics of simulated data with 5 M reads. Read length distribution (left) and read count distribution by genes in log scale (right). B,C Summary statistics between true and estimated isoform expressions using LIQA (blue), FLAIR (red), Mandalorion (gray), TALON (green), and Oxford Nanopore Pipeline (ONP) (yellow) at different read coverages. B Spearman’s correlations (left) and RMSE (right) between estimated and true TPM. C Spearman’s correlations (left) and RMSE (right) between estimated and true relative abundance