Fig. 2
From: Long-read cDNA sequencing identifies functional pseudogenes in the human transcriptome
Deletion of PDCL3P4 impacts the transcriptome of haploid cells. a PDCL3P4 is a pseudogene transcribed in HAP1 cells from the canonical long terminal repeat (LTR) promoter of an upstream human endogenous retrovirus-K (HERV-K) sequence. Grey bars within the PacBio reads represent exons and light blue bars represent introns. b CRISPR-Cas9 genome engineering removes the retroposed portion of PDCL3P4 from the HAP1 genome in three independent clones. c PDCL3P4 expression is ablated in PDCL3P4 mutant clones. d PDCL3P4 ablation disrupted the expression of more than 137 genes, while PDCL3 expression was unaffected. e PDCL3P4 transcripts were enriched in the nucleus. The nuclear-localised noncoding RNA MALAT1 and mRNA ACTB act as controls