Fig. 4
From: PEPPRO: quality control and processing of nascent RNA profiling data
Library complexity is measured with unique read frequency distributions and projections. a Schematic demonstrating PCR duplication and library complexity (dashed line represents completely unique library). b Library complexity traces plot the read count versus externally calculated deduplicated read counts. Deduplication is a prerequisite, so these plots may only be produced for samples with UMIs. Inset zooms to region from 0 to double the maximum number of unique reads. c The position of curves in panel b at a sequencing depth of 10 million reads (dashed line represents minimum recommended percentage of unique reads)