Fig. 3

RNA integrity is assessed with degradation ratios and insert sizes. a Schematic illustrating intact versus degraded libraries. b Degradation ratio for test samples (HelaS3 GRO sample could not be calculated; Values less than dashed line (1.0) are considered high quality). c–f Insert size distributions for: c, a degraded single-end library; d, a degraded paired-end library; e, a non-degraded single-end library; and f, a non-degraded paired-end library (orange shading represents highly degraded reads; yellow shading represents partially degraded reads)