Fig. 6
HDAC4 is required to load a repressive complex in correspondence to well-defined AP-1 regulated SES that antagonizes p300. a Analysis of SA-β-gal positivity in SK-LMS-1 WT cells expressing NeoR and in SK-LMS-1/HDAC4−/− cells re-expressing the indicated transgenes. n = 3. The significance is relative to KO cells expressing NeoR. b AP-1 transcriptional activity in SK-LMS-1 WT cells expressing NeoR and in SK-LMS-1/HDAC4−/− cells re-expressing the indicated transgenes. n = 3. The significance is relative to KO cells expressing NeoR. c Heat-map of the H3K27ac levels in WT and HDAC4−/− cells in a window of 40 kb around the analyzed SES. The TSS, the SES, and the regions analyzed by ChIP-qPCR are indicated. d mRNA expression levels of the indicated genes in SK-LMS-1 WT cells expressing NeoR and in SK-LMS-1/HDAC4−/− cells re-expressing the indicated transgenes. n = 3. The significance is relative to KO cells expressing NeoR. e ChIP-qPCR signals, normalized to total H3, obtained for the indicated antibodies in the indicated cells. Mean ± SD; n = 3. The significance is relative to KO cells expressing NeoR. f mRNA expression levels of the indicated genes in BJ/hTERT/RAS cells re-expressing or not HDAC4 and E1A, as indicated, in respect to BJ/hTERT/NeoR cells. The significance is relative to BJ/hTERT/NeoR cells. g ChIP-qPCR signals for the indicated loci in BJ/hTERT/RAS cells re-expressing or not HDAC4 and E1A, as indicated, in respect to BJ/hTERT/NeoR cells. The significance is relative to BJ/hTERT/RAS cells