Fig. 5

Inducible enhancers are regulatory hubs. a Uniform Manifold Approximation and Projection (UMAP) of scATACseq profiles of LNCaP cells treated with either EtOH or DHT. Each dot represents an individual cell (EtOH n = 7857; DHT = 6661). b chromVAR deviation score enrichment of different AR enhancer classifications was compared to random genomic regions in the UMAP. c The chromatin accessibility of pseudo-bulk scATACseq at each AR enhancer class compared to random genomic regions. d Change in the median number of co-accessible sites for each AR enhancer class following androgen treatment. e A representative network graph of the CREs of BMPR1B shows changes in co-accessibility following androgen treatment. The gained inducible AR enhancer led to significant increases in network complexity. f The relative impact on network complexity following DHT treatment was calculated for all ARBS in each AR enhancer class. Inducible enhancers had the most significant impact on network complexity leading to higher co-accessibility with other ARBS (p < 10⁻⁶)