Fig. 1

Generation of PD-L1-lnc in PD-L1 protein-positive or PD-L1 protein-negative LUAD via alternative splicing. a Agarose gel analysis and Sanger sequencing of the PCR end-product in PD-L1 protein-positive or PD-L1 protein-negative tumor tissues. b Upper, the schematic of primers for amplification of PD-L1 mRNA and PD-L1-lnc; Lower, the agarose gel and Sanger sequencing of the 878-bp and 705-bp band obtained by RT-PCR. c RNA BaseScope for PD-L1 mRNA (blue) and PD-L1-lnc (red) in PD-L1 protein-positive and PD-L1 protein-negative tumor tissues. Scale bar, 20 μm. d Upper, the schematic of primers for amplification of PD-L1 mRNA and PD-L1-lnc; Lower, the expression level of PD-L1 mRNA and PD-L1-lnc in PD-L1 protein-positive or -negative tumor tissues by qRT-PCR with specific probes. NS, no significance