Fig. 5

Functional screens of 4088 variants in BRCA1/2 using base editors recognizing “NGN” PAM sequence. a Schematic overview of the base editing screens with “NGN” base editors and efficiency correction. b Editing efficiencies for AncBE4max-NG and ABEmax-NG base editors measured by the genome-integrated target site library assay. The orange line represents median of the fraction of edited reads for all sgRNAs in the library. c–f Histograms of score distributions for variants tested in four screens: BRCA1-AT-NGG (c), BRCA1-CG-NGG (d), BRCA2-AT-NGG (e), BRCA2-CG-NGG (f). g Fraction of loss-of-function variants among all the tested variants in different mutational categories