Fig. 4
From: ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics
Comparison of ACME and formaldehyde as cell fixation reagents. a Experimental workflow of trypsin dissociation with ACME and formaldehyde fixation. b Flow cytometry ungated and gated profiles of trypsin-dissociated ACME-fixed cells stained with DRAQ5 (nucleus) and Concanavalin-A (cytoplasm). c Bioanalyzer profiles and RIN values of RNA samples dissociated with trypsin and fixed with ACME or increasing concentrations of formaldehyde (FA). Inferred values (Inf RIN, blue open boxes) are calculated from a correlative analysis of the % area of the two ribosomal bands compared to the total. As a control sample, we used RNA from worms dissociated with trypsin and introduced unfixed in TRIzol