Fig. 2

ADAR1 transcript undergoes m6A modification near the STOP codon in GBM. a Box plot showing METTL3 and METTL14 mRNA expression in 163 GBMs (red plot) and 207 normal brain cortex tissues (blue plot) (http://gepia2.cancer-pku.cn), * p ≤ 0.05. b Immunohistochemical analysis performed on 10 GBM tissues and human brain cortex (two representative pictures of GBMs and control are shown). c Schematic representation of ADAR1 mRNA, with details of the RNA sequence near the stop codon (TAG). In red, the m6A consensus sequence within ADAR1 mRNA. Two different sets of primers for ADAR1 amplification are shown, with a thin (PCR 1) and a thick (PCR 2) black line. d qRT-PCRs (PCR 1 and 2) of ADAR1 RNA fragments in U87MG (left) and U118MG (right) cells after m6A immunoprecipitation (n = 3). HPRT expression was used as negative control. e–f m6A immunoprecipitation of ADAR1 mRNA (PCR 1 and 2) and control HPRT upon METTL3 silencing (n = 3). Values are represented as means ± SD, *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001