Fig. 2
From: Decontamination of ambient RNA in single-cell RNA-seq with DecontX
Contamination in a human-mouse cell mixture dataset. a The total number of UMIs aligned specifically to the mouse or human genome is plotted for each droplet. b The proportion of counts for mouse genes in human cells is highly correlated to the average expression of these genes across all mouse cells indicating that the amount of contamination for each gene is proportional to how highly that gene is expressed in the contaminating cell population. c Similarly, the proportion of counts for human genes in the mouse cells is highly correlated to the average expression of those genes across all human cells. d While each droplet is predicted to contain a single cell, the median percentage of contamination for human and mouse cells is 1.09% and 2.75%, respectively. The range of contamination is 0.43–45.09% indicating the need for contamination estimation for each individual cell