Fig. 5

Factors that determine knockout efficiency in single-cell CRISPR screens. a The knockout effect on target gene downregulation (measured by negative selection p value) and the median target expression in mESC dataset. b The negative selection p values of all targets in naïve and primed states in mESC CROP-seq dataset. c, d The expression of Tdh in Tdh knockout cells and other cells in naïve and primed states. The CD46 gene is marked as red. e The fraction of target genes with the strongest p values, where target gene is ranked top ten among all other genes, in high MOI and low MOI conditions. f, g The p value (f) and selection score (g) of all possible genes upon CD46 knockdown in K562 dataset. CD46 itself is marked as red