Fig. 5

Microexon protein-protein interaction network. a PPI network using as input genes that have microexons that are differentially included across mouse embryonic brain development. Edges represent protein-protein interactions that are supported by either experimental evidence or databases. Edge width represents the confidence of the interaction based on a combined score calculated by STRING. Colours represent different Reactome pathways that were enriched on the network. b Eigencentrality calculated for each gene node in relation to the developmental stage at which each microexon was included. Labels are provided for the protein nodes belonging to the upper quartile eigencentrality, and their colours indicate their corresponding annotation status. c Effect of microexon alternative splicing for different Reactome pathways. Counts indicate the number of microexons that start to be differentially included at each developmental stage. d, e Eigencentrality and earliest developmental stage at which each gene is affected by differential microexon inclusion show differences across some of the GO categories that were significantly enriched after gene background correction. Statistical differences were assessed by one-sided Wilcoxon test while correcting for multiple comparisons. Significant p values are denoted by *> 0.05, **> 0.01 and ***> 0.001