Fig. 4

Inclusion properties of microexon clusters. a Number of microexons belonging to each cluster. b Mean loading factors across each cluster for PC1 and PC2. c Mean and standard deviation of PSI values across microexon clusters. d Mean U2 scores and in-frame fraction across microexon clusters. e Mean PSI values across neuronal and neuromuscular microexons. Each grey line represents the mean PSI values for a microexon across all samples from a tissue cluster or neuronal developmental stage (x-axis). Colour lines represent the mean PSI values across all clusters and stages. f–h Alternative microexons detected between non-neuronal tissue samples and midbrain, hindbrain and neural tube (f); forebrain (g); and adrenal gland (AG), heart (HRT) and skeletal muscle (SKM) (h). Microexon splicing changes are the percentage of microexons corresponding to each microexon cluster, where microexon inclusion fractions are represented with blue bars and exclusion events with red bars. i Intersection between microexon sets that were differentially included across sample groups. The vertical bars show the number of microexons corresponding to combinations indicated by the connected dots below. j Area-proportional Euler diagram representing the most abundant intersections between differentially included microexon sets