Fig. 6

Reproductive 24PHAS precursors are widely cleaved by miR2275 on membrane-bound polysomes in maize and rice. a, c Abundance of different miR2275 members in input (Total), TP, and MBP samples from maize immature tassels (a) and rice immature panicles (c). b, d Number (left panels) and overlap of 24PHAS precursors cleaved by miR2275 (right panels) in Total, TP, and MBP samples from maize immature tassels (b) and rice immature panicles (d). Cleavage sites with category = 0, which means that the reads with the maximum count (> 1) are mapped to only one indicated position on the transcript, and P value ≤ 0.05 in at least two biological repeats were filtered as miR2275 target sites. e, f Abundance of reproductive 24-nt phasiRNAs in Total, TP, and MBP samples from maize immature tassels (e) and rice immature panicles (f). Abundance of miR2275 and reproductive 24-nt phasiRNAs (a, c, e, and f), and the number of target 24PHAS precursors (left panels of b and d) are displayed as mean ± standard deviation (SD) of three biological repeats. “RPMR” is short for “reads per million rRNA fragments” (a, c, e, and f). Comparisons between MBP and TP (a and c) and between Total, TP, and MBP (left panels of b and d, e and f) were performed by two-tailed unpaired t-test, and P values are displayed to the right of the corresponding MBP miR2275s (a and c) or above the corresponding comparisons (left panels of b and d, e and f)