Fig. 3

Enrichment of miRNAs on membrane-bound polysomes in maize and rice. a, c Size and abundance of all miRNAs in various samples from maize immature tassels (a) and rice immature panicles (c). b, d Identification of differentially accumulated miRNAs between TP and input (Total) (left panels), and between MBP and TP (right panels) in maize immature tassels (b) and rice immature panicles (d). The cutoff parameters for differentially accumulated miRNAs are fold change ≥ 2 and P value ≤ 0.05. “Group I,” “Group II,” “Group III,” and “Group IV” represent “miRNAs that were polysome-depleted,” “miRNAs enriched on polysomes,” “miRNAs that were associated with polysomes but MBP-depleted,” and “miRNAs that were MBP-enriched,” respectively. e Abundance of representative miRNAs (ZmmiR390a/b-5p, ZmmiR528a/b-5p, ZmmiR529-5p, ZmmiR529-3p, OsmiR390-5p, and OsmiR528-5p) in Total, TP, and MBP samples from maize immature tassels and rice immature panicles. miRNA abundance is displayed as mean ± standard deviation (SD) (a, c, and e) or the mean of three biological repeats (b and d). “RPMR” is short for “reads per million rRNA fragments.” f Northern blotting verification for miRNAs in (e) with Total, TP, and MBP RNA preparations that were used for sRNA library construction. 5S rRNA was used as an internal control, and U6 was used as a nuclear RNA marker