Fig. 2

Characterization of isoform-enriched genes in cell lines. a The number of cell lines that expressed isoforms in common. b The number of cell lines that expressed genes with isoforms in common. c–e The distribution of lengths (c), exons (d), and expression level (e) of genes with isoforms in VMRC-LCD. Isoform-enriched genes showed tendencies of shorter length, fewer exons, and higher expression. **P < 0.01 and ***P < 0.001 (Kruskal–Wallis test and Dunn–Bonferroni’s post hoc test). f The results of the gene ontology enrichment analysis for genes with at least one isoform among cell lines. g Comparisons of the distribution of the number of isoforms in cell lines with or without the driver mutations EGFR, KRAS, and NRAS. *P < 0.05 (Welch’s t-test). h Comparison of the number of isoforms and TMB for each cell line. No significant correlation was observed (r = 0.30)