Fig. 1

Discovery of evCpGs. a |Δβ|^{MZ twin pairs} and |Δβ|^{unrelated pairs} distributions in an example of an evCpG (left) and a variably methylated non-evCpG (right). Similarity p values were obtained via equivalence testing with a two one-sided tests procedure. b Venn-Euler diagram displaying significant hits across three normalization methods (StrQN, dasen and oob_RELIC_QN_BMIQ), where the intersection between the three sets corresponds to evCpGs. c Manhattan plot displaying CpG significance across chromosomes (odds and even represented either in blue or orange), where evCpGs are highlighted in green. For each CpG, we used the maximal p value across three normalization methods. d Agreement between twins measured as concordance plotted against methylation range (see Additional file 3: Supplementary Methods for details), where evCpGs are highlighted in blue