Fig. 4
From: An integrated multi-omics approach to identify regulatory mechanisms in cancer metastatic processes
Knockdown of JunD impairs cell migration and invasion. a Immunoblot of JunD and α-tubulin in lysates from SW480 (M0) cells expressing JunD shRNA knockdown (shJunD) or scramble control (shScr). b Migration for JunD knockdown (blue line) and scramble control (Scr; red line) was monitored continuously over 40 h using a xCelligence realtime cell analysis platform with cell invasion migration (CIM) plates. Fetal bovine serum was used as a chemoattractant. Cell index (arbitrary units) corresponds to cell migration capacity. Dotted lines represent the standard deviation (SD) of three independent cultures measured in parallel. c Cell invasiveness was measured continuously for 60 h using CIM plates that were precoated with Matrigel. All other parameters are the same as for b