Fig. 2

Diagram of the terminal regions of the genomes of the old smallpox vaccines VK01, VK02, VK05, VK08, and VK12. The genomes of the VK vaccines, horsepox MNR-76, Mulford 1902 vaccine, VACV-IOC_B141, and Dryvax clone DPP13 were aligned using the Geneious alignment plugin in Geneious Prime and visualized in CLC Main Workbench. A schematic figure was created based on the alignment showing the left (a) and the right (b) termini of the genomes. Minor deletions and gaps were omitted. For sake of clarity, insertions in the genomes of VK01, VK12, Mulford 1902, VACV-IOC_B141, and VACV-DPP13 that split the 10.7- kb sequence of horsepox genome in two parts were removed and are shown as in insert on the bottom (green dotted line) of a. The green dotted arrow indicates the region of the alignment from which the insertions were spliced out in each genome. The red dotted boxes indicate the regions of the virus genomes in which the 10.7 kb (a) and 5.5 kb (b) sequences of horsepox virus (thick black lines) are present or absent. The stars indicate the ITR junction sites in virus genomes