Table 3 List of primers used for RT-(q)PCR analysis
From: Intronic tRNAs of mitochondrial origin regulate constitutive and alternative splicing
Target | Primer fwd | Primer rev |
|---|---|---|
eGFP mRNA | TGAGGAGGCTTTTTTGGAGG | TTCACTAATCGAATGGATCTGTC |
eGFP pre-mRNA | GTAATACGACTCACTATAGGGC | CATCAATATCCCAAGGAGCATG |
Beta-Actin | CGTCACCAACTGGGACGACA | CTTCTCGCGGTTGGCCTTGG |
DsredExpress2 | GTCCTTCCCCGAGGGC | TTCAGCACGCCGTCGCG |
GAPDH | CCATGGGGAAGGTGAAGGTC | AGTTAAAAGCAGCCCTGGTGA |
Alt. spl. Rep. | AGTGATTCAGAACCGTCAAG | TCCACCACCGTCTTCTTTAG |
PPFIBP1 incl ex 29 | ccaaagtgaagCCAAAGAAACTT | aatcttccatctgctctaaccg |
PPFIBP1 excl ex 29 | gttctagagcctcgttttaacg | tgaatcttccatcttcactttgg |
PPFIBP1 upstream | gaaacagaaaaagagacagcaga | CTTCTCCTAAGTtttccaaagagt |