Table 1 List of primers used for classical cloning in this study.
From: Intronic tRNAs of mitochondrial origin regulate constitutive and alternative splicing
Plasmid | Primer fwd | Primer rev | Entry plasmid |
|---|---|---|---|
Y | GGGGTACCCCGTTCCG ATATCTTTGTGATTG | GGAATTCCATATGGAA TTCCCACCTTAAGACCT CTGGTA | Low0-eGFP |
YC | GGGGTACCCCGTTCCG ATATCTTTGTGATTG | GGAATTCCATATGGAA TTCCTCTACTTCTACCG CCGAAA | Low0-eGFP |
YCN | GGGGTACCCCGTTCCG ATATCTTTGTGATTG | GGAATTCCATATGGAATTCCAGACCTCAACTAGATTGGC | Low0-eGFP |
YCNA | GGGGTACCCCGTTCCG ATATCTTTGTGATTG | GGAATTCCATATGGAATTCCAACTTCTGATAAGGACTGTAG | Low0-eGFP |
(nucl.) YCNAW | GGGGTACCCCGTTCCG ATATCTTTGTGATTG | GGAATTCCATATGGAATTCCGCTGTCATAAGTACAATAACC | Low0-eGFP/Low2-eGFP |
C | GGGGTACCCCTTTTTACCAGAGGTCTTAAGG | GGAATTCCATATGGAA TTCCTCTACTTCTACCG CCGAAA | Low0-eGFP |
N | GGGGTACCCCCTACCGCCATTTTTTTTTTCG | GGAATTCCATATGGAA TTCCAGACCTCAACTAG A TTGGC | Low0-eGFP |
A | GGGGTACCCCGCCAATCTAGTTGAGGTCT | GGAATTCCATATGGAATTCCAACTTCTGATAAGGACTGTAG | Low0-eGFP |
W | GGGGTACCCCCTACAGTCCTTATCAGAAGTT | GGAATTCCATATGGAATTCCGCTGTCATAAGTACAATAACC | Low0-eGFP |