Fig. 2

Efficiency evaluation of Drosophila codon-optimized Cas13 variants. a–d qPCR analysis showing eCFP transcript levels in Sg4 cells as a function of the different Cas13 variants that were expressed in these cells (a–d, respectively). Shown are relative fold changes of eCFP transcript being targeted by two independent crRNAs, crRNA 1 (red) and crRNA 2 (cyan). Data were normalized to eCFP expression levels when using a blank crRNA as a control (blue dotted line = 1). * = p value < 0.05, ** = p value < 0.01, *** = p value < 0.001, ns = not significant, p values based on Student’s t test, error bars represent 95% confidence intervals. e Fluorescence changes of eCFP across samples targeted by the Cas13/crRNA 2 complex. Fluorescence levels were measured using ImageJ and normalized to signals obtained with a blank crRNA (control). Nuclei were stained with Nuclear Green LCS1 (ab138904). Color was adjusted for color-blind-friendly purpose. eCFP and DsRed fluorescence were measured using their native fluorescence properties (no antibody staining). Scale bar = 50 μm