Fig. 2

POL2A ensures silencing independently of ATXR5/6-mediated H3K27me1. a Overlap between TEs upregulated in atxr5/6 (data from Ikeda et al. [35]) and pol2a-12. b Metaplots showing average H3K27me1 enrichment (log₂ signal over input) at H3K27me1 peaks. Shaded areas show standard deviation. One biological replicate is shown. c Metaplots showing H3K27me1 changes (log₂ mutant / WT) pol2a-12 and atxr5/6 (data from Ma et al. [43]) at H3K27me1 peaks overlapping TEs upregulated in both atxr5/6 and pol2a-12, represented as in b. Average of two replicates is shown. d Transcript accumulation in reads per kilobase per million mapped reads (RPKM) in indicated genotypes. The effect of genotype was verified with a Kruskal-Wallis rank-sum test. Significant differences between groups, evaluated by a Dwass-Steel-Crichtlow-Fligner test, are indicated by lowercase letters (P < 0.05). Two biological replicates are shown for each genotype, except for pol2a atxr5/6 where only one sample was analyzed