Fig. 4

Gene expression analysis in PBMCs of SLE patients with and without DAP1 risk. a Schematic for the RNA sequencing experiment in PBMCs of established SLE patients. RNA was extracted from PBMCs from SLE patients with rs2930047 protective genotype (TT, n = 6) or donor with rs2930047 risk genotype (CC, n = 10). b Heatmap shows the top two clusters of most differentially expressed 232 genes (RPKM values) between DAP1 SLE risk and non-risk allele. c, d Top 10 biological pathways annotated for each cluster based on PANTHER 14.1 classification (http://pantherdb.org). e Heatmap on 54 sub-selected genes to illustrate the status of some key biological molecules from the immune system, autophagy, and apoptosis pathways. f, g Expression plot of FCRLA and DAP1 gene, as representative genes from each cluster