Fig. 2

EDAL inhibits viral replication in neuronal cells. a N2a cells were transfected with pcDNA3.1 or pcDNA-EDAL, then at 12 h post transfection, the cells were infected with RABV at MOI 0.01 and virus titers were measured at indicated time points. b N2a cells were transfected with three different sets of EDAL-specific siRNAs (siEDAL-①, ②, ③). At 24 h post transfection, the cells were infected with RABV at MOI 0.01 and virus titers were measured at indicated time points. c N2a cells were transfected with siEDAL or siNC (negative control) for 8 h and then transfected with pcDNA3.1 or pcDNA-EDAL. At 12 h post transfection, the cells were infected with RABV at MOI 0.01 for 24 h and virus titers in the cell supernatant were measured. d N2a cells were transfected with pcDNA3.1 or pcDNA-EDAL, then at 12 h post transfection, the cells were infected with VSV at MOI 0.01 and virus titers were measured at indicated time points. e N2a cells were transfected with pcDNA3.1 or pcDNA-EDAL, then at 24 h post transfection, the cells were infected with SFV at MOI 0.01 and virus titers were measured at indicated time points. f N2a cells were transfected with pcDNA3.1 or pcDNA-EDAL, then at 12 h post transfection, the cells were infected with HSV-1 at MOI 0.01 and virus titers were measured at indicated time points. g, h EDAL and reverse EDAL (revEDAL) were inserted into the genome of a recombinant RABV (rRABV), named rRABV-EDAL and rRABV-revEDAL respectively (g), and their growth kinetics in N2a cells (MOI = 0.01) were compared (h). i N2a cells were infected with rRABV, rRABV-EDAL, or rRABV-revEDAL at MOI 0.005 for 48 h and the viral spread was compared by calculating the cell numbers within the fluorescence focus. Scale bar, 50 μm. Statistical analysis of grouped comparisons was carried out by Student’s t test(*P < 0.05;**P < 0.01; ***P < 0.001). Bar graph represents means ± SD, n = 3