Fig. 2
From: Natural display of nuclear-encoded RNA on the cell surface and its impact on cell interaction
Imaging flow cytometry (IFC) analysis of maxRNAs co-localization with immune cell surface markers in human PBMCs. a A schematic view of the extracellular hybridization of the fluorophore (red dot) labeled probes (isFISH probes) to a hypothetical maxRNA. b, c IFC images of two representative PBMC cells (rows) in 5 channels (columns), including DAPI, CD19, CD3ε, CD14, and maxRNA probes, as well as merged images of all channels (Composite). d, e The proportions of PBMCs that exhibit IFC signals (y-axis) when assayed with maxRNA probes and 3 types of controls (dArt4 probe, 6-mer library, and fluorophore only). Each box plot was derived from 4 independent biological replicates. d Total IFC positive cells PBMCs for each probe treatment. e CD14+, CD3ε+, CD19+, and CD3ε−CD14−CD19− PBMCs are separately plotted