Fig. 3

Small non-coding RNA annotation in AalbF2. a Summary statistics on annotated piRNA clusters using the genome assemblies for Ae. aegypti AaegL5 and Ae. albopictus AaloF1 or AalbF2. b Size distribution of piRNA clusters annotated with the old AaloF1 assembly or the most recent AalbF2 assembly. The density plot shows the number of clusters normalized to the total number of piRNA clusters. c Enrichment of repeat classes or nrEVEs in piRNA clusters compared to the whole genome. d log2 piRNA coverage on an exemplary uni-strand (left panel) or dual-strand (right panel) piRNA cluster (given as piRNAs per million mapped reads [rpm]). Annotated genes are indicated with arrows, repeat features, and nrEVEs with gray or red boxes for positive or negative strands, respectively. e miRNA abundance in Ae. albopictus carcass and ovary samples. Counts for individual miRNAs were normalized to the total number of miRNAs in each dataset and expressed as log2-transformed reads per million miRNAs (rpmm) + 1. The mean of two independent libraries for each condition is shown. The highly abundant miR-34 and selected miRNAs with high expression in the ovary, but not carcass, are indicated. f Fold induction of miRNA levels in blood-fed ovary samples compared to sugar-fed samples. The basal expression of each miRNA in the sugar-fed samples is indicated in gray scale. Only miRNAs with an induction ≥ 5-fold are shown. Color coding in a and b represents the basis for the miRNA prediction, as indicated