Fig. 4
Genomic correlates of MA-TAM and its association with cellular origination and polarization state. a Kaplan-Meier survival curve analysis of 373 GBM patients based on MA-TAM signature score. b Genomic landscape of 133 MA-TAMhigh and MA-TAMlow tumors (left panel). Somatic mutations, including single-nucleotide variations (SNVs), and small insertions/deletions, and copy number alterations, are shown. All somatic mutations with an allele frequency of > 5% are shown. Percentage of altered cases in key GBM driver pathways (right panel). c tSNE analysis of 3589 single-cell transcriptomes. Cell clusters are differentially colored and annotated based on each distinct cellular compartment (left panel). Expression of cell-type-specific signatures, polarization state, microenvironmental state overlaid on the tSNE space (right panel). d MA-TAM signature scores (upper panel) and master regulator activity (lower panel) in IVY Glioblastoma Atlas Project (IGAP) dataset. Cellular Tumor (CN; n = 111), hyperplastic blood vessels (HBV; n = 22), microvascular proliferation (MVP, n = 28), pseudo-palisading cells around necrosis (PCAN, n = 40), peri-necrotic zone (PNZ, n = 26), infiltrating tumor (IT, n = 24), and leading edge (LE, n = 19). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001