Fig. 2

Characterization of sgCBEs derived from various MS2 sgRNAs. a Cartoon representation of the Cryo-EM structure of SpCas9 sgRNA and NTS. The location of each loop of sgRNA was shown in red. b SpCas9 sgRNA sequence and schematic diagram of MS2-modified sgRNA scaffold. c Efficiency of cytosine editing with various MS2-sgRNA derived base editors. HEK293T cells were transfected with plasmids expressing BE3 or sgCBE that targeted a set of 12 different sites. C-to-T editing efficiencies were analyzed by Sanger sequencing and EditR calculating. Each experiment was repeated at least three times. Data are represented as mean ± SEM. d Heat map showing the base editing window of various base editors. Four sites that harbored multiple Cs, including site A, site B, VEGFA site2, and SHANK3, were used to summarize the editing window, and the average C-to-T conversion rate of the indicated potion was calculated. Each experiment was repeated at least three times. Data are represented as mean ± SEM