Fig. 3
SERBP1 binding motif. a SERBP1 RNA binding motif obtained with RNACompete. b Fluorescence polarization assay shows SERBP1’s high affinity (KD ~ 47 nM) to a 7-mer RNA oligonucleotide (5′- GCGCGGG - 3′). c ~ 40% of transcripts determined via RIP-Seq as preferentially associated with SERBP1 display the identified GC-rich motif in their 3′ UTR, a number much higher than expected by chance. d Results of luciferase assay showing that co-transfection of a SERBP1 expression vector increased the expression of reporter constructs containing the 3′ UTR of genes displaying putative SERBP1 binding motifs. GAPDH was used as a negative control