Fig. 6

Analysis of tissue-specific methylation patterns. a Distribution of TEs according to pattern score in A. thaliana samples from seedling and specific tissues. b Separation of TEs in A. thaliana wild type tissues according to read and among-site CHH methylation variation. c, d Distribution of TEs according to pattern score in A. thaliana vegetative nucleus (c) and sperm (d) wild type samples, alongside drm1/2 and cmt2 mutants of the respective tissues. e, f Correlation between pattern score of individual TEs in wild type compared to mutant tissues. Only elements that were methylated in both wild type and respective mutant were selected. Each dot represents a single TE, with the distance from dashed line (f(x)=x) indicating a shift in pattern score between samples (i.e. change in the average CHH enzyme methylating the element). g Schematic representation of the methylation patterns of CMT2 and DRM2 identified in this study: CMT2-mediated methylation has higher stochasticity, possibly reflecting lower processivity, and a unimodal distribution among cells; DRM2-mediated methylation has lower stochasticity, possibly reflecting higher processivity, and has a bimodal distribution among cells