Fig. 4

HIPPO framework applied to 10K E18 mouse heart cells. a Distributions of means across gene features selected by Seurat, Hippo, both, or none. b Sequential feature selection visualizes how genes gradually align closer to the expected Poisson line as more heterogeneity accounted for. c Heatmaps of top features selected at the first 5 rounds of clustering. d Top differential expression genes obtained at each round of clustering. e Visualization of sequential clustering using t-SNE plots