Fig. 2
Reprogramming of chromatin structure during pig in vitro fertilized early embryogenesis. a Schematic of generation of IVF, PA, and AG embryos (left) and microscopy images of 1-cell, 4-cell, and morula embryos staining with Hoechst (right). b Normalized Hi-C contact heatmap of 10 Mb region in chromosome 3 were shown at 50 kb resolution in the three stages of IVF embryos and PEF. c The accumulative curve for genome coverage by domains size. X-axis represents the relative domain size, i.e., domain length/chromosome length. Only domains larger than 1 MB were included in this figure. The P values are given for the t test of average domain sizes. d PC1 and correlation matrix of chr14 at 100 kb resolution. e Observed/expected (O/E) aggregate plot of TADs in pig and mouse. f The contact frequency decay curves of Hi-C data during pig (upper) and mouse (bottom) embryogenesis. g Compartment strength which were calculated as AA*BB/AB2 for each chromosome in embryos of pig (upper) and mouse (bottom). Compartments identified in morula and 8-cell stages were used as a reference for pig and mouse, respectively. h Mean value of insulation scores around PEF TAD boundaries (upper) and MEF TAD boundaries (bottom). The color code is identical to f. *P < 0.05, **P < 0.01, ^P < 0.1