Fig. 5

Gene expression patterns in the female gametes revealed by scRNA-seq analysis. a Clustering analysis of female gamete-expressed genes in LCM datasets [64, 65] and in scRNA-seq data (this study) (upper panel) and new sets of female gamete-expressed genes (lower panel) identified by scRNA-seq analysis. Gene expression levels in scRNA-seq were mean transcript per million (TPM) values of the genes in all cells for each cell type. EC, egg cell; CC, central cell; SC, synergid cell. b Venn diagram showing the numbers of the genes that are expressed in three female gametophytic cells. An expressed gene is defined as its expression in one or more cells examined. c Expression clustering of all CRP (cysteine-rich peptides) genes in EC, CC, and SC. d Fractions of CPR transcripts out of total mRNA transcripts in EC, CC, and SC