Fig. 4
From: Sierra: discovery of differential transcript usage from polyA-captured single-cell RNA-seq data
3 ′UTR shortening in activated and proliferating cardiac fibroblasts following MI. a, b UMAP visualisation of fibroblast populations from Pdgfra-GFP +/CD31 − mouse cardiac cells at 3 days post-sham or MI surgery showing a an aggregate of all cells and b the UMAP plot separated according to condition. c–e Counts of 3 ′UTR peaks showing differential usage according to their relative location to the terminating exon. Location of 0 indicates the peak most proximal to the terminating exon, with 1 representing the most distal. Comparisons performed are for c F-Cyc against F-SL and F-SH combined, d F-CI against F-SL and F-SH combined, and e F-Act against F-SL and F-SH combined. f, g Relative expression of peaks most distal and proximal (to terminating exon) for fTimp2 and gCd47 as visualised on UMAP coordinates. h, i Read coverage across 3 ′UTR for select single-cell fibroblast populations from sham (F-SL/F-SH combined) and MI (F-Act, F-CI, F-Cyc) datasets compared to bulk RNA-seq of FACS-sorted fibroblasts from sham and MI conditions for hTimp2 and iCd47