Fig. 10

Differential DNA methylation for differentially expressed genes in mature pollen. a MET (left) and JSD (right) profiles (arithmetic mean, 2-kb flanks, 200-bp bins) from sperm cells for high- (top 50) and low-expressed (bottom 50) genes in mature pollen. The top panels show the metagene profiles over all top and bottom 50 genes, respectively. The bottom panels show profiles of each gene as a heatmap. b A genome browser view centered at a gene in the bottom 50 expressed group in mature pollen (myb-like HTH transcriptional regulator family protein; AT2G01060). The TE track highlights the TE family (blue), the superfamily (grey), and the targeting DNA methylation pathway (red) if applicable. The signal tracks are for JSD (scatter plot) and MET (heatmaps) in sperm cells using context-dependent colors as in the rest of the text. The eFP image shows relative gene expression of AT2G01060 and was generated with the “Tissue Specific Microgametogenesis eFP” of ePlant [48] using Affymetrix ATH1 array data [49]