Fig. 2

The compartments and TADs do not notably change after RNA polymerase depletion. a Saddle plots representing compartmentalization strength (left), average insulation score displayed for the CTCF-bound and CTCF-unbound TAD boundaries, from Bonev et al. [44] (middle); heat map of the average observed/expected Hi-C interactions in the TAD regions (right) under untreated and Pol I-degraded conditions (24 h). b Illustrated as a, but with Pol II degradation Hi-C data sets. c Illustrated as a, but with Pol III degradation Hi-C data sets. d Violin plots showing quantification of the aggregated intra-TAD observed/expected contact enrichment values for various RNAP-degraded conditions based on the BAT Hi-C data presented in a–c. Compared to that upon CTCF depletion, there was no significant change in these chromatin structures 6 h or 24 h after RNAP degradation. p values were calculated using a two-sided Wilcoxon test. e Averaged insulation score displayed for the CTCF-bound and CTCF-unbound TAD boundaries from Bonev et al. [44] (left), and heat map of the average observed/expected Hi-C interactions in the TAD regions (right) under untreated and CTCF-degraded conditions (2 days). f Illustrated as a, but with Pol II degradation during mitotic exit in Hi-C data sets