Fig. 6

Identification of the pathologic targets. a Schematic illustration of the identification of the targets of pathologic antibodies. b Representative illustration of the proteins that interacted with pathologic antibodies. c Validation of the proteins bound by the pathologic antibodies. d The macrophages in the microenvironment of the NSCLC mainly belong to the M2 macrophages. The expression of M2 macrophage-specific marker CD163 and MRC1 (CD206) in the single-cell dataset was plotted. e Overexpression of AP2A1 results in increased endocytosis of IgGs. Left panel shows the experimental design. A549 cells were transduced with AP2A1 overexpression or vector plasmid and treated with IgG. Cellular IgGs were determined by western blot (right panel). f A549 cell lines were electroporated with PBS (ctrl), pathology RHOC IgGs, and RHOC-IgG depleted IgGs, and the whole cell lysates were harvested 3 h after electroporation. The expression levels of RHOC and TRIM21 were determined by western blot. GAPDH was used as the internal control. g A549 cell lines were electroporated with PBS (ctrl) or recombinant rabbit antibodies against RHOC, and the whole cell lysates were harvested 3 h after electroporation. The expression levels of RHOC and TRIM21 were determined by western blot. GAPDH was used as the internal control. h Schematic of TRIM21-mediated degradation of specific targets in tumor cells