Table 2 Multiplex genome editing using SWISSv3 in T0 rice plants
Editing events | Target sites | Genotype of mutations | Efficiencies |
|---|---|---|---|
Single | OsALS-T2 | C6>T6 (1); C6C7>T6T7 (1) | 3.6% (2/55) |
OsACC-T2 | A4>G4 (2) | 3.6% (2/55) | |
OsBADH2-Indels | deletions (12); insertions (4) | 29.1% (16/55) | |
Double | OsALS-T2/OsACC-T2 | C6>T6+A4>G4 (1) | 1.8% (1/55) |
OsALS-T2/OsBADH2-Indels | C6>T6+deletion (2); C6>T6+insertion (1); C6>G6+deletion (1); C6C7>T6T7+deletion (2); C6C7C16>T6T7T16+insertion (1) | 12.7% (7/55) | |
OsACC-T2/OsBADH2-Indels | A4>G4+deletion (1); A7>G7+deletion (1) | 3.6% (2/55) | |
Triple | OsALS-T2/OsACC-T2/OsBADH2-Indels | C6>T6+A4>G4+ deletion (1); C6>G6+ A4>G4+ insertion (1); C6>T6+A7>G7+ deletion (1); C6C7>T6T7+ A4>G4+ deletion (1) | 7.3% (4/55) |