Table 2 Several variables related to the collection and evaluation of microbial communities by amplicon sequencing have been performed. This table presents a subset of relevant variables and references to the studies in which they have been evaluated

Use of preservative agents (cryoprotectants, RNAlater, etc.)

[116,117,118,119,120,121,122,123]

Sequence database

[124,125,126,127,128]

Sequencing platform

[116, 125, 129,130,131]

Sample storage

[117,118,119, 127, 129, 132,133,134,135,136,137,138,139,140,141,142]

DNA extraction method (comparison of kits)

[116, 125, 127, 129, 130, 132, 140, 143,144,145,146,147,148]

Lysis method (chemical, enzymatic, mechanical)

[143,144,145, 149,150,151]

Sample collection (method, sampling location, sampler)

[118, 126, 129, 131, 134, 150, 152,153,154,155,156]

16S rRNA gene variable region sequenced

[10, 14, 124]