Fig. 6

Elavl1a regulates maternal RNA stability in a structure-dependent fashion. a Design of the GFP reporter mRNA with single-stranded or double-stranded Elavl1a binding site in its 3′ UTR. b The structure models of designed (i) wild-type, (ii) mutant, and (iii) rescue Elavl1a binding sites and flanking regions. c The relative mRNA level (6 h.p.f. versus 0.5 h.p.f.) of reporter genes with different structural contexts of Elavl1a binding motif in control and elavl1a morphants, n = 3 for each group, error bars, mean ± s.d.; P values were determined by two-sided Student’s t test. d The protein level of reporter gene with different structural contexts of Elavl1a binding motif at 6 h.p.f. in control and elavl1a morphants determined by GFP fluorescence signal observation. Some pictures of representative embryos were shown. Quantitative fluorescence intensity was shown on the right, n = 20 for each group. Error bars, mean ± s.d. P values were determined by two-sided Student’s t test. e Schematic model shows that Elavl1a regulates RNA stability in a structure-dependent fashion