Fig. 1

Comprehensive RNA structural maps during zebrafish early embryogenesis. a Schematic view of in vivo RNA structural maps during zebrafish early development using icSHAPE. b Nucleotide composition in transcriptome and all profiled sites (transcriptome, A: 27.91%, U: 25.96%, C: 22.47%, G: 23.66%; icSHAPE, A: 27.86%, U: 25.99%, C: 22.37%, G: 23.78%). c The number of transcripts with more than half of the nucleotides with valid structural signals at each stage. d Global structural changes by violin plot of average icSHAPE reactivity of each transcript during zebrafish early development; P values were calculated by paired two-sided Student’s t test. e Integrative Genomics Viewer (IGV) view of icSHAPE reactivity and RNA structure model of kpna4 gene at 3′ UTR region