Fig. 3

Hi-D maps single-cell biophysical properties of genome conformation and behavior. a Diffusion constant spatially mapped onto the nucleus of a quiescent cell (left) and the same nucleus upon serum stimulation b Spatial distribution of populations found by the GMM for the diffusion constant for quiescent (left) and actively transcribing cells (right). c Relative share of populations on the cell volume (n = 12). Numbers in percent. d For each population in b, the relative share of chosen MSD models is represented as a stacked histogram in the quiescent state (left bars) and actively transcribing state (right bars). e Spatial distribution of populations for the anomalous exponent for quiescent (left) and actively transcribing cells (right). f Detailed insight into the spatial patterning of the low population of the anomalous exponent. The intermediate and high population is shown in light and dark red respectively