Fig. 2

TADsplimer successfully detected TAD splits. a Chromosome map showing the genomic locations of fibroblast (IMR90) TADs that were split in HUVEC (blue) and HUVEC TADs that were split in IMR90 (red). b Heatmaps showing the average frequency of chromatin interaction in 6 aggregates: merged in HUVEC (top left) and split in IMR90 (top right), split in HUVEC (middle left) and merged in IMR90 (middle right), and all adjoint TADs in HUVEC (bottom left) and IMR90 (bottom right). c Violin plot of TAD sizes for merged, split, and regular TADs from HUVEC and IMR90 cells. d Boxplots showing the binding frequency of CTCF at the individual group of TAD boundaries. e Heatmaps showing the number of split TADs between cell types. f Boxplots showing the Jaccard index of split TADs between replicates. Results were plotted for individual TAD split identification methods. g Boxplots showing the Jaccard index of identified TADs between replicates. Results were plotted for individual TAD calling methods. h Enrichment of representative pathways in genes associated with split or merged TADs. P value was determined by Fisher’s exact test and adjusted by the B-H method. i Heatmaps and barplot showing the enriched pathways and the number of enriched pathways, respectively, for genes in split and merged TADs defined by alternative methods. P value was determined by Fisher’s exact test and adjusted by the B-H method